Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Anais da Academia Brasileira de Ciências

Patrícia M.o. Pierre²  Saulo M. Sousa¹  Lisete C. Davide¹  Marco A. Machado¹  Lyderson F. Viccini¹ 

[1] ,Universidade Federal do Espírito Santo Centro de Ciências Humanas e Naturais Departamento de Ciências BiológicasVitória ES ,Brasil

关键词: cheotype;    cytogenetic;    cytotype;    DNA content;    RAPD;    Lippia alba;    quimiotipo;    citogenética;    citótipo;    conteúdo de DNA;    RAPD;    Lippia alba;   

DOI  :  10.1590/S0001-37652011005000012

来源: SciELO

【 摘 要 】

Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of ixoploidy. ur data indicates that sexual reproduction aong those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.

【 授权许可】

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