Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

Anais da Academia Brasileira de Ciências

Claudia R. De Rainho¹  Andréa Kaezer¹  Claudia A.f. Aiub¹  Israel Felzenszwalb¹ 

[1] ,Universidade do Estado do Rio de Janeiro Instituto de Biologia Roberto Alcantara Gomes Departamento de Biofísica e BiometriaRio de Janeiro RJ ,Brasil

关键词: Allium cepa;    genotoxicity;    mutagenicity;    N-nitrosodiethylaine;    Tradescantia pallida var. purpurea;    Allium cepa;    genotoxicidade;    mutagenicidade;    N-nitrosodietilamina;    Tradescantia pallida var. purpurea;   

DOI  :  10.1590/S0001-37652010000400015

来源: SciELO

【 摘 要 】

N-nitroso compounds, such as N-nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH) using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM) genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water) and positive control maleic hydrazide (MH 30mg/mL). After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI) and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM), showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dose-dependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.

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