Evaluation of semi-automated cells counting in peritoneal fluid

Jornal Brasileiro de Patologia e Medicina Laboratorial

Daikelly I. Braghirolli¹  Patricia Pranke¹  Luciane N. Calil¹ 

关键词: peritoneal fluid;    ascites;    cell count;    body fluids;    líquido peritoneal;    ascite;    contagem de células;    fluidos corpóreos;   

DOI  :  10.5935/1676-2444.20150037

来源: SciELO

【 摘 要 】

ABSTRACTIntroduction:Currently, the cytological analysis of biological fluids, such as peritoneal fluid, is performed by manually cells counting in Fuchs-Rosenthal chamber. However, this method has a number of limitations. Because of these limitations, automatic counters have been evaluated for cell counting in this type of sample in order to make it faster and more reliable test.Objective:The aim of this study is to compare the manual and semi-automated leukocytes and erythrocytes counting in peritoneal fluid.Materials and methods:The samples were analyzed manually and using the CountessTM(Invitrogen).Results:The results showed that although there is a correlation between the two counting methods, the correlation is relatively low, for both leukocytes and erythrocytes analysis.Conclusion:The results suggest that peritoneal fluid should continue to be analyzed in Fuchs-Rosenthal chamber. However, further studies should be conducted with a greater number of samples to investigate the possibility of using automated cells counting in serous fluids and, thus, provide greater speed and quality of results.

【 授权许可】

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