Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates

Jornal Brasileiro de Patologia e Medicina Laboratorial

Antonio Marcio Barbosa Junior²  Bruno Fernandes De Oliveira Santos¹  Erick De Oliveira Carvalho¹  Dângelly Lins Figuerôa Martins De Mélo¹  Rita De Cássia Trindade¹  Maria Aparecida De Resende Stoianoff¹ 

[1] ,Universidade Federal de Sergipe

关键词: Cryptococcus neoformans;    fungal proteins;    mycology;    yeast;    Cryptococcus gattii;    Cryptococcus neoformans;    proteínas fúngicas;    micologia;    leveduras;    Cryptococcus gattii;   

DOI  :  10.1590/S1676-24442013000300002

来源: SciELO

【 摘 要 】

INTRODUCTION: Cryptococcus neoformans and Cryptococcus gattii are encapsulated basidiomycetous yeasts with worldwide distribution. They cause cryptococcosis with features of systemic infection, affecting the central nervous system, lungs and skin in humans and animals. These fungi present numerous virulence factors that allow them to invade the host and multiply, among which extracellular enzyme capacity and microbial adaptation to different temperatures are worth mentioning. OBJECTIVE: To evaluate the production of protease and investigate possible differences in thermotolerance and urease activity in clinical and environmental yeast isolates. MATERIAL AND METHODS: Culture methods and Pz analysis were applied to assess urease and protease, whereas the optical density method was used to analyze biological activity in thermotolerance. RESULTS: There was no significant results as to microbial growth at the tested temperatures (25º, 37º and 42ºC). It was observed that clinical specimens grew better than environmental ones at elevated temperatures. As to C. neoformans, the moderate production of urease enzyme prevailed in both clinical and environmental isolates within 24h or 48h. Moreover, there was significant production on the seventh day of reading. The best reading time for viewing protease production in both isolates and species was the seventh day: 96% clinical samples and 94% environmental isolates. CONCLUSION: Further studies are required in order to investigate the virulence factors of C. neoformans and C. gattii cerebrospinal isolates from patients with meningoencephalitis and environmental samples from Sergipe. Furthermore, a higher technical accuracy and statistical precision are indispensable.

【 授权许可】

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