DNA measurements after radiation-induced tissue structure of thyroid gland of rats

Jornal Brasileiro de Patologia e Medicina Laboratorial

Roberto Souza Camargo²  Neuza Kasumi Shirata¹  Eliana Ogassavara Setani¹  Eduardo Anselmo Garcia¹  Eduardo Pompeu¹  Eduardo Martella¹  Celso Di Loreto¹  Adhemar Longatto Filho¹ 

[1] ,Faculdade de Medicina da Universidade de São Paulo Faculdade de Medicina Departamento de Cirurgia

关键词: Thyroid cancer;    Irradiation;    DNA content;    Câncer tireóideo;    Irradiação;    Conteúdo de DNA;   

DOI  :  10.1590/S1676-24442005000300006

来源: SciELO

【 摘 要 】

INTRODUCTION: Thyroid gland exposures to radiation induce nuclear chromosomal alteration. Objective: To evaluate the DNA content of thyroid gland submitted to radiation. MATERIALS AND METHODS: We radiated 75 rats while 25 were not radiated to be used as control group. Exposure was conducted by the use of Cobalt-60 radioactive source in the right anterior cervical region in a field of 3-30cm, comprising the second and the sixth tracheal rings with 600-centigray (cGY) doses. The DNA content (ploidy) was obtained with Feulgen-thionin stain and was quantified with CAS 200 quantitative measurement equipment. RESULTS: Diploid pattern was obtained in 88 cases (95.7%), independently of time of exposure: on the other hand, aneuploidy was observed in four cases (4.3%) only in the group sacrificed to the 33 days. Eight cases were excluded due to technical reasons. CONCLUSION: The early aneuploid pattern found in our study certainly corroborated that radiation affects thyroid gland with important consequences in terms of disorders.

【 授权许可】

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