ITS-2 sequences-based identification of Trichogramma species in South America

Brazilian Journal of Biology

R. P. Almeida¹  R. Stouthamer¹ 

关键词: molecular markers;    ITS-2 sequence;    Hymenoptera;    egg parasitoid;    marcadores moleculares;    sequências ITS-2;    Hymenoptera;    parasitóide de ovos;   

DOI  :  10.1590/1519-6984.04614

来源: SciELO

【 摘 要 】

Abstract ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License