期刊论文详细信息
Brazilian Journal of Biology
Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus
Jd Biller-takahashi1  Hj Montassier1  Ls Takahashi1  Ec Urbinati1 
关键词: fish;    methodology;    immunology;    acquire immunology;    humoral immunity;    peixe;    metodologia;    imunologia;    imunidade adquirida;    imunidade humoral;   
DOI  :  10.1590/1519-6984.21312
来源: SciELO
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【 摘 要 】

Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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