Nuclear activity in Apis mellifera L. (Hymenoptera, Apidae) queen ovary cells demonstrated by silver nitrate impregnation and ultrastructure

Brazilian Journal of Biology

C. Cruz-landim²  K. Patrício¹ 

[1] ,Universidade Estadual Paulista Instituto de Biociências de Rio Claro Departamento de BiologiaRio Claro SP ,Brazil

关键词: nucleolar;    ovary;    RNP;    vitellogenesis;    nucléolos;    ovário;    RNP;    vitelogênese;   

DOI  :  10.1590/S1519-69842010000500023

来源: SciELO

【 摘 要 】

The present paper shows through conventional light microscopy, silver nitrate impregnation and Transmission Electron Microscopy, the changes in nuclear activity that occurs in the ovary cells during vitellogenesis of Apis mellifera (Linnaeus, 1758). The material impregnated by silver nitrate was detected in nurse cells, oocyte germinal vesicle and follicular cells, whose amounts are greater in phases corresponding to ovarian cells more intense activity. The silver impregnation permitted to demonstrate that the oocyte germinal vesicle synthezises ribonucleoproteins (RNP) during the beginning of the vitellogenesis. The ultrastructure show nucleolar-like material corresponding to nuclear sites impregnated by silver.

【 授权许可】

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