Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Brazilian Journal of Biology

V. Gobatto¹  Sg. Giani¹  M. Camassola¹  Ajp. Dillon¹  A. Specht¹  Nm. Barros¹ 

[1] ,Universidade de Caxias do Sul Centro de Ciências Agrárias e Biológicas Instituto de BiotecnologiaCaxias do Sul RS ,Brazil

关键词: Anticarsia gemmatalis;    Bacillus thuringiensis;    Cry proteins;    Culex quinquefasciatus;    isolation;    Anticarsia gemmatalis;    Bacillus thuringiensis;    proteínas Cry;    Culex quinquefasciatus;    isolamento;   

DOI  :  10.1590/S1519-69842010000500018

来源: SciELO

【 摘 要 】

Samples of the Bacillus thuringiensis (Bt) were collected from soil and insects. Eight isolates were selected from rural soil, 15 from urban soil and 11 from insects. These were evaluated for entomopathogenicity against larvae of Anticarsia gemmatalis and Culex quinquefasciatus. The pathogenicity tests showed that a higher percentage of isolates were active against A. gemmatalis (60%) compared to C. quinquefasciatus (31%). Probit analysis (LC50) indicated that against A. gemmatalis four of the isolates presented values similar to the reference strain against A. gemmatalis, while against C. quinquefasciatus one isolate showed an LC50 similar to the reference strain (IPS-82). SDS-PAGE characterisation of two isolates showed a 27 kDa protein fraction related to the Bt subspecies israelensis cytolytic toxin (cyt) gene. One 130 kDa protein, possibly related to the Bt crystal inclusions (cry1) gene, was identified in the other two isolates, which were more toxic for lepidoptera; another isolate presented a protein of 100 kDa. Some new local Bt isolates had similar LC50 probit values to the reference strains.

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