In vivo anti-angiogenic effects further support the promise of the antineoplasic activity of methyl jasmonate

Brazilian Journal of Biology

Jef. Pereira Lopes²  Mr. Barbosa²  Cn. Stella²  Wa. Santos²  Em. Pereira²  J. Nogueira-neto¹  Em. Augusto²  Lv. Silva²  Ss. Smaili¹  Lf. Gomes² 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas e ToxicológicasSão Paulo SP ,Brazil

关键词: methyl jasmonate;    antiangiogenesis;    CAM model;    endothelial cell culture;    HUVEC;    metiljasmonato;    antiangiogênese;    modelo da CAM;    cultura de células endoteliais;    HUVEC;   

DOI  :  10.1590/S1519-69842010000200029

来源: SciELO

【 摘 要 】

Molecular plant components have long been aimed at the angiogenesis and anti-angiogenesis pathways, and have been tested as sources for antineoplasic drugs with promising success. The present work deals with the anti-angiogenic effects of Methyl Jasmonate. Jasmonate derivatives were demonstrated to selectively damage the mitochondria of cancer cells. In vitro, 1-10 mM Methyl Jasmonate induced the cell death of the human umbilical vein endothelial cells (HUVEC) and the Murine melanoma cells (B16F10), while micromolar concentrations were ineffective. In vivo, comparable concentrations were toxic and reduced the vessel density of the Chorioallantoic Membrane of the Chicken Embryo (CAM). However, 1-10 µM concentrations produced a complex effect. There was increased capillary budding, but the new vessels were leakier and less organised than corresponding controls. It is suggested that not only direct toxicity, but also the drug effects upon angiogenesis are relevant to the antineoplasic effects of Methyl Jasmonate.

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