Leukocytes respiratory burst and lysozyme level in pacu (Piaractus mesopotamicus Holmberg, 1887)

Brazilian Journal of Biology

Js. Abreu²  Cm. Marzocchi-machado¹  Ac. Urbaczek¹  Lm. Fonseca¹  Ec. Urbinati¹ 

[1] ,Universidade Federal do Mato Grosso Faculdade de Agronomia e Medicina Veterinária Departamento de Zootecnia e Extensão RuralCuiabá MT ,Brazil

关键词: pacu;    Piaractus mesopotamicus;    innate immunity;    respiratory burst;    lysozyme;    pacu;    Piaractus mesopotamicus;    imunidade inata;    burst oxidativo;    lisozima;   

DOI  :  10.1590/S1519-69842009000500018

来源: SciELO

【 摘 要 】

Innate immune responses are useful to determine the health status of fish and to evaluate the effect of immunomodulatory substances in fish farming. Leukocytes respiratory burst was measured in pacu (Piaractus mesopotamicus) using chemiluminescence assay and nitroblue tetrazolium (NBT) reduction assay. The nitroblue tetrazolium reduction seemed more adequate than chemiluminescence assay for leukocytes oxidative burst determination, since it was difficult to isolate the blood leucocytes for chemiluminescence assay. Plasma and serum lysozyme were measured using a turbidimetric assay. The heating of serum and plasma samples (56 ºC for 30 minutes) for complement system inactivation darkened the plasma samples and interfered in the results. The lysozyme activity in serum was higher than in plasma, suggesting that serum samples are more appropriate for the analysis. This study established protocols that can be useful tools in the study of immune mechanisms of the tropical fish pacu.

【 授权许可】

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