Molecular variations in Vibrio alginolyticus and V. harveyi in shrimp-farming systems upon stress

Brazilian Journal of Microbiology

Anix Vivek Santhyia¹  Rosalind George Mulloorpeedikayil¹  Riji John Kollanoor¹  Prince M.j. Jeyaseelan¹ 

关键词: Vibrio harveyi;    Vibrio alginolyticus;    molecular variation;    shrimp;    Penaeus monodon;   

DOI  :  10.1590/S1517-838246420140410

来源: SciELO

【 摘 要 】

A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and gyrB gene specific PCR. The bacterial strains were genotyped by PCR fingerprinting using GTG(5) and IS (Insertion Sequence) primers. Seven strains each of V. alginolyticus and V. harveyi were subjected to 10 passages through trypticase soya broth (TSB), which contained different NaCl concentrations (3, 6 and 8%) and trypticase soya agar (TSA). V. alginolyticus was also passaged through TSB with a 12% NaCl concentration. PCR fingerprinting, which was performed on the strains that were passaged through different salt concentrations, confirmed that V. alginolyticus and V. harveyi could affect the genomic variations, depending on the environmental conditions of the culture. The study highlights the complex genotypic variations that occur in Vibrio strains of tropical aquatic environment because of varied environmental conditions, which result in genetic divergence and/or probable convergence. Such genetic divergence and/or convergence can lead to the organismal adaptive variation, which results in their ability to cause a productive infection in aquatic organisms or generation of new strains.

【 授权许可】

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