Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli

Brazilian Journal of Microbiology

Franciane Gomig¹  Carolina Weigert Galvão¹  Denis Leandro De Freitas¹  Larissa Labas¹  Rafael Mazer Etto¹  Luiz Antonio Esmerino¹  Marcelo Andrade De Lima¹  Marcia Helena Appel¹  Silvio Marques Zanata¹  Maria Berenice Reynaud Steffens¹  Helena Bonciani Nader¹  Rafael Bertoni Da Silveira¹ 

关键词: ODC;    gyrA;    parC;    uropathogenic;   

DOI  :  10.1590/S1517-838246320131291

来源: SciELO

【 摘 要 】

Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic E. coli strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic E. coli strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region gyrA and parC mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative E. coli isolates.

【 授权许可】

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