期刊论文详细信息
Brazilian Journal of Microbiology
GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium
Qun Luo1  Ying He1  Deng-yong Hou1  Jian-guo Zhang1  Xian-rong Shen1 
关键词: alkane hydroxylase;    expression;    Escherichia coli DH5α;    biodegradation;    diesel oil;   
DOI  :  10.1590/S1517-838246320120226
来源: SciELO
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【 摘 要 】

To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in E. coli DH5α could enhance the function of diesel oil degradation by the bacterial consortium.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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