Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats

Brazilian Journal of Microbiology

Thais Sebastiana Porfida Ferreira¹  Maria Roberta Felizardo¹  Debora Dirani Sena De Gobbi¹  Marina Moreno¹  Andrea Micke Moreno¹ 

关键词: cat;    Pasteurela multocida;    virulence genes;    resistance;    isolation;   

DOI  :  10.1590/S1517-838246120140084

来源: SciELO

【 摘 要 】

Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P. multocida, and forty-one strains were selected and characterized by means of biochemical tests and PCR. The P. multocida strains were tested for capsular type, virulence genes and resistance profile. A total of 75.6% (31/41) of isolates belonged to capsular type A, and 24.4% (10/41) of the isolates were untypeable. None of the strains harboured toxA, tbpA or pfhA genes. The frequencies of the other genes tested were variable, and the data generated were used to build a dendrogram showing the relatedness of strains, which were clustered according to origin. The most common resistance profile observed was against sulfizoxazole and trimethoprim-sulphamethoxazole.

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