Bacterial diversity in relatively pristine and anthropogenically-influenced mangrove ecosystems (Goa, India)

Brazilian Journal of Microbiology

Sheryl Oliveira Fernandes²  David L. Kirchman¹  Valérie D. Michotey¹  Patricia C. Bonin¹  P.a. Lokabharathi² 

[1] ,National Institute of Oceanography Biological Oceanography Division Dona Paula Goa ,India

关键词: bacteria;    denitrification;    diversity;    mangroves;    sediments;   

DOI  :  10.1590/S1517-83822014000400006

来源: SciELO

【 摘 要 】

To appreciate differences in benthic bacterial community composition at the relatively pristine Tuvem and the anthropogenically-influenced Divar mangrove ecosystems in Goa, India, parallel tag sequencing of the V6 region of 16S rDNA was carried out. We hypothesize that availability of extraneously-derived anthropogenic substrates could act as a stimulatant but not a deterrent to promote higher bacterial diversity at Divar. Our observations revealed that the phylum Proteobacteria was dominant at both locations comprising 43-46% of total tags. The Tuvem ecosystem was characterized by an abundance of members belonging to the class Deltaproteobacteria (21%), ~ 2100 phylotypes and 1561 operational taxonomic units (OTUs) sharing > 97% similarity. At Divar, the Gammaproteobacteria were ~ 2x higher (17%) than at Tuvem. A more diverse bacterial community with > 3300 phylotypes and > 2000 OTUs mostly belonging to Gammaproteobacteria and a significantly higher DNT (n = 9, p < 0.001, df = 1) were recorded at Divar. These findings suggest that the quantity and quality of pollutants at Divar are perhaps still at a level to maintain high diversity. Using this technique we could show higher diversity at Divar with the possibility of Gammaproteobacteria contributing to modulating excess nitrate.

【 授权许可】

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