Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

Brazilian Journal of Microbiology

Judit Süle²  Tímea Kõrösi¹  Attila Hucker¹  László Varga² 

[1] ,University of West Hungary Faculty of Agricultural and Food Sciences Institute of Food ScienceMosonmagyaróvár,Hungary

关键词: mupirocin;    clindamycin;    ciprofloxacin;    Bifidobacterium;    Lactobacillus acidophilus;   

DOI  :  10.1590/S1517-83822014000300035

来源: SciELO

【 摘 要 】

The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp.

【 授权许可】

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