Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa

Brazilian Journal of Microbiology

Ilana Schneider Lima¹  Nicole Carmo Baumeier¹  Rosimeire Takaki Rosa¹  Patrícia Maria Stuelp Campelo¹  Edvaldo Antonio Ribeiro Rosa¹ 

[1] ,Pontifícia Universidade Católica do Paraná Escola de Saúde e Biociências Curitiba PR ,Brazil

关键词: Pseudomonas aeruginosa;    glyphosate;    growth;    biofilm;   

DOI  :  10.1590/S1517-83822014000300029

来源: SciELO

【 摘 要 】

This study evaluated the impact of different concentrations of glyphosate (Rondup®) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC®15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD560nm every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p > 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC®15442.

【 授权许可】

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