Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex

Brazilian Journal of Microbiology

Clarissa Frota Macatrão Costa¹  Valério Monteiro Neto²  Bruno Rafael De Carvalho Santos²  Bruno Rafael Rabelo Costa¹  Alexandre Azevedo²  Josilene Lima Serra²  Hermínio Benítez Rabello Mendes²  Adenilde Ribeiro Nascimento²  Mariana Bonfim Pinto Mendes²  Oliver Kuppinger² 

[1] ,Universidade Federal do MaranhãoSão Luís MA ,Brazil

关键词: microbiological analysis;    coliforms;    enterobacterias;    ballast water;    Escherichia coli;   

DOI  :  10.1590/S1517-83822014000300026

来源: SciELO

【 摘 要 】

The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia coli, Proteus mirabilis, Citrobacter freundii, Proteus vulgaris, Klebsiella pneumoniae, Klebsiella ozaenae, Morganella morganii, Enterobacter cloacae and Edwardsiella tarda. Out of the 51 E. coli isolated, two were positive for the elt gene and one was positive for the CVD432 sequence, features of enterotoxigenic and enteroaggregative strains, respectively. This study reveals that the PCM is contaminated by enterobacteria and diarrheagenic E.coli thus providing evidence regarding the risk of these bacteria being carried by ships to other countries, and draws attention to the input of fecal bacteria brought by ships in the port waters of Maranhão.

【 授权许可】

CC BY-NC   
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