Production of humic substances through coal-solubilizing bacteria

Brazilian Journal of Microbiology

Nelson Valero²  Liliana Gómez²  Manuel Pantoja²  Ramiro Ramírez¹ 

[1] ,Universidad Popular del Cesar Laboratory of Environmental and Agricultural Microbiology Valledupar,Colombia

关键词: low rank coal;    Microbacterium sp;    Bacillus mycoides;    Acinetobacter baumannii;    Enterobacter aerogenes;   

DOI  :  10.1590/S1517-83822014000300021

来源: SciELO

【 摘 要 】

In this paper, the production of humic substances (HS) through the bacterial solubilization of low rank coal (LRC) was evaluated. The evaluation was carried out by 19 bacterial strains isolated in microenvironments with high contents of coal wastes. The biotransformed LRC and the HS produced were quantified in vitro in a liquid growth medium. The humic acids (HA) obtained from the most active bacterial strain were characterized via elemental composition (C, H, N, O), IR analyses, and the E4/E6 ratio; they were then compared with the HA extracted chemically using NaOH. There was LRC biotransformation ranged from 25 to 37%, and HS production ranged from 127 to 3100 mg.L-1. More activity was detected in the isolated strains of Bacillus mycoides, Microbacterium sp, Acinetobacter sp, and Enterobacter aerogenes. The HA produced by B. mycoides had an IR spectrum and an E4/E6 ratio similar to those of the HA extracted with NAOH, but their elemental composition and their degree of aromatic condensation was different. Results suggest that these bacteria can be used to exploit the LRC resulting from coal mining activities and thus produce HS in order to improve the content of humified organic matter in soils.

【 授权许可】

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