In vitro activity of Amazon plant extracts against Enterococcus faecalis

Brazilian Journal of Microbiology

Adriana Lígia De Castilho²  Juliana Paola Correa Da Silva²  Cintia Helena Coury Saraceni²  Ingrit Elida Collantes Díaz¹  Mateus Luís Barradas Paciencia¹  Antonio Drauzio Varella¹  Ivana Barbosa Suffredini² 

[1],Universidade Paulista Programa de Graduação em Odontologia São Paulo SP ,Brazil

关键词: Enterococcus faecalis;    endodontitis;    antibacterial activity;    Amazon rain forest;    bioprospection;   

DOI  :  10.1590/S1517-83822014000300002

来源: SciELO

【 摘 要 】

Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC® 29212TM and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 µg/mL), Diclinanona calycina (MIC < 40 µg/mL) and Moronobea coccinea (40 < MIC < 80 µg/mL; MBC = 80 µg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by 13C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.

【 授权许可】

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