Ethanol production from agricultural wastes using Sacchromyces cervisae

Brazilian Journal of Microbiology

Muhammad Irfan¹  Muhammad Nadeem¹  Quratualain Syed¹ 

[1] ,Pakistan Council of Scientific and Industrial Reasearch Laboratories Complex Food & Biotechnology Research Center Ferozpure Road Lahore,Pakistan

关键词: lignoccellulosic biomass;    Pretreatment;    Sacchromyces cervisae;    ethanol production;   

DOI  :  10.1590/S1517-83822014000200012

来源: SciELO

【 摘 要 】

The main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by Sacchromyces cervisae. All the three substrates were ground to powder form (2 mm) and pretreated with 3%H2O2 + 2% NaOH followed by steaming at 130 °C for 60 min. These substrates were hydrolyzed by commercial cellulase enzyme. The whole fermentation process was carried out in 500 mL Erlenmeyer flask under anaerobic conditions in submerged fermentation at 30 °C for three days of incubation period. FTIR analysis of the substrates indicated significant changes in the alteration of the structure occurred after pretreatment which leads to efficient saccharification. After pretreatment the substrates were hydrolyzed by commercial cellulase enzyme and maximum hydrolysis was observed in sugarcane bagasse (64%) followed by rice straw (40%) and wheat straw (34%). Among all these tested substrates, sugarcane bagasse (77 g/L) produced more ethanol as compared to rice straw (62 g/L) and wheat straw (44 g/L) using medium composition of (%) 0.25 (NH4)2SO4, 0.1 KH2PO4, 0.05 MgSO4, 0.25 Yeast extract by S. cervisae.

【 授权许可】

CC BY-NC   
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