Canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1: effects of inoculation and aeration rate

Brazilian Journal of Microbiology

Forouzan Rostami²  Seyed Hadi Razavi²  Abbas Akhavan Sepahi¹  Seyed Mohammad Taghi Gharibzahedi² 

[1] ,University of Tehran Faculty of Agricultural Engineering and Technology Department of Food Science & EngineeringKaraj,Iran

关键词: Dietzia;    microbial canthaxanthin;    batch bioreactor;    response surface methodology;    modeling;   

DOI  :  10.1590/S1517-83822014005000046

来源: SciELO

【 摘 要 】

The interest in production of natural colorants by microbial fermentation has been currently increased. The effects of D-glucose concentration (3.18-36.82 g/L), inoculum size (12.5 x 10(9)-49.5 x 10(9) cfu cells/mL) and air-flow rate (1.95-12.05 L/L min) on the biomass, total carotenoid and canthaxanthin (CTX) accumulation of Dietzia natronolimnaea HS-1 in a batch bioreactor was scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD). Second-order polynomial models with high R² values ranging from 0.978 to 0.990 were developed for the studied responses using multiple linear regression analysis. The models showed the maximum cumulative amounts of biomass (7.85 g/L), total carotenoid (5.48 mg/L) and CTX (4.99 mg/L) could be achieved at 23.38 g/L of D-glucose, 31.2 x 10(9) cfu cells/mL of inoculation intensity and air-flow rate of 7.85 L/L min. The predicted values for optimum conditions were in good agreement with experimental data.

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