Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis

Brazilian Journal of Microbiology

Tatiana Reis Do Rosário²  Cristina Corsi Dib¹  Eliana Roxo¹  Sônia Regina Pinheiro²  Silvio Arruda Vasconcellos²  Nilson Roberti Benites² 

[1] ,Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia São Paulo SP ,Brazil

关键词: tuberculosis;    Mycobacterium bovis;    thin layer;    PCR;    cattle and buffalo organs;   

DOI  :  10.1590/S1517-83822014005000038

来源: SciELO

【 摘 要 】

The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).

【 授权许可】

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