Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings

Brazilian Journal of Microbiology

A. Dayanandan¹  S. Hilda Vimala Rani¹  M. Shanmugavel¹  A. Gnanamani¹  G. Suseela Rajakumar¹ 

[1] ,Central Leather Research Institute Department of Microbiology New Delhi

关键词: wheat bran;    oil cakes;    lipase;    tannery fleshing;    fat hydrolysis;   

DOI  :  10.1590/S1517-83822013000400010

来源: SciELO

【 摘 要 】

The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.

【 授权许可】

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