Brazilian Journal of Microbiology | |
Yeast biomass production: a new approach in glucose-limited feeding strategy | |
Érika Durão Vieira2  Maria Da Graça Stupiello Andrietta1  Silvio Roberto Andrietta1  | |
[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Campinas SP ,Brazil | |
关键词: yeast; biomass; fed-batch; process simulation; Saccharomyces cerevisiae; | |
DOI : 10.1590/S1517-83822013000200035 | |
来源: SciELO | |
【 摘 要 】
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses feeding on the basis of a simple theoretical model in which has taken into account the nutritional growth needs of yeast cell and two input data: the theoretical specific growth rate and initial cell biomass. In experimental assay, a commercial baker's yeast strain and molasses as sugar source were used. Experimental results showed an overall biomass yield on substrate of 0.33, a biomass increase of 6.4 fold and a specific growth rate of 0.165 h-1 in contrast to the predicted value of 0.180 h-1 in the second stage simulation.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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