Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process

Brazilian Journal of Microbiology

Ranjan Singh²  Rajeeva Gaur¹  Soni Tiwari¹  Manogya Kumar Gaur¹ 

[1] ,Amity University Amity Institute of Microbiol Biotechnology Noida,Índia

关键词: Aureobasidium pullulans;    Pullulan;    Non-stirred fed-batch fermentation;   

DOI  :  10.1590/S1517-83822012000300030

来源: SciELO

【 摘 要 】

Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.

【 授权许可】

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