Occurrence of killer Candida glabrata clinical isolates

Brazilian Journal of Microbiology

Arroyo-helguera O²  De Las Penas Alejandro¹  Castaño Irene¹ 

[1] ,Universidad Veracruzana Instituto de Salud Pública Xalapa Veracruz ,México

关键词: Candida glabrata;    killer activity;    yeast;    Saccharomyces;   

DOI  :  10.1590/S1517-83822012000300005

来源: SciELO

【 摘 要 】

In this work we characterized the occurrence of killer activity in 64 Candida glabrata clinical isolates under different conditions. We found that only 6.25 % of the clinical isolates tested were positive for killer activity against a Saccharomyces cerevisiae W303 sensitive strain. Sensitivity of killer activity to different values of pH and temperatures was analyzed. We found that the killer activity presented by all isolates was resistant to every pH and temperature tested, although optimal activity was found at a range of pH values from 4 to 7 and at 37ºC. We did not observe extrachromosomal genetic elements associated with killer activity in any of the positive C. glabrata isolates. The killer effect was due to a decrease in viability and DNA fragmentation in sensitive yeast.

【 授权许可】

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