Interspecific variation of the bacterial community structure in the phyllosphere of the three major plant components of mangrove forests

Brazilian Journal of Microbiology

Armando Cavalcante Franco Dias²  Rodrigo Gouveia Taketani¹  Fernando Dini Andreote¹  Danice Mazzer Luvizotto¹  João Luis Da Silva¹  Rosely Dos Santos Nascimento¹  Itamar Soares De Melo¹ 

[1] ,Universidade de São Paulo Centro de Energia Nuclear na Agricultura Piracicaba SP ,Brasil

关键词: culture-independent profiling;    plant genotype;    surface leaves;   

DOI  :  10.1590/S1517-83822012000200030

来源: SciELO

【 摘 要 】

Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species (Avicenia shaueriana, Laguncularia racemosa and Rhizophora mangle), found in two mangroves. The number of bacteria in the phyllosphere of these plants varied between 3.62 x 10(4) in A. schaeriana and 6.26 x 10³ in R. mangle. The results obtained by PCR-DGGE and isolation approaches were congruent and demonstrated that each plant species harbor specific bacterial communities in their leaves surfaces. Moreover, the ordination of environmental factors (mangrove and plant species), by redundancy analysis (RDA), also indicated that the selection exerted by plant species is higher than mangrove location on bacterial communities at phyllosphere.

【 授权许可】

CC BY-NC   
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