Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

Brazilian Journal of Microbiology

Fernando Luiz Tobias²  Luize Néli Nunes Garcia²  Milton Masahiko Kanashiro¹  Enrique Medina-acosta¹  João Gato Brom-de-luna²  Claudia Maria Costa De Almeida²  Romildo Rocha Azevedo Junior¹  Môsar Lemos¹  Olney Vieira-da-motta² 

[1] ,Universidade Estadual do Norte Fluminense Laboratório de Sanidade Animal Campos dos Goytacazes RJ ,Brasil

关键词: Ostrich IgY antibodies;    inhibition;    recombinant proteins;    E. coli;    S. aureus;   

DOI  :  10.1590/S1517-83822012000200015

来源: SciELO

【 摘 要 】

Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two subsequent immunization steps with 21 days intervals were repeated in 0,85% saline in FIA. Blood and eggs samples were collected before and after immunization steps. Egg yolk immunoglobulins were purified by precipitation with 19% sodium sulfate and 20% ammonium sulphate methodologies. Purified IgY 50µL aliquots were incubated in 850µL BHI broth containing 50µL inoculums of five strains of S. aureus and five strains of E.coli during four hours at 37ºC. Growth inhibition was evaluated followed by photometry reading (DO550nm). Egg yolk IgY preparation from hiperimmunized birds contained antibodies that inhibited significantly (p<0,05) growth of strains tested. Potential use of ostrich IgY polyclonal antibodies as a diagnostic and therapeutic tool is proposed for diseased animals.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License