Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Brazilian Journal of Microbiology

Carla R. Santos¹  Laura G. Franciscatto¹  Regina B. Barcellos²  Sabrina E. M Almeida²  Maria Lucia R. Rossetti¹ 

[1] ,Universidade Luterana do BrasilCanoas RS ,Brasil

关键词: HPV;    cervical cancer;    FTA elute;    molecular detection;   

DOI  :  10.1590/S1517-83822012000100047

来源: SciELO

【 摘 要 】

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

【 授权许可】

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