Isolation and characterization of potential lactic acid bacteria (LAB) from freshwater fish processing wastes for application in fermentative utilisation of fish processing waste

Brazilian Journal of Microbiology

Jini R²  Swapna Hc²  Amit Kumar Rai²  Vrinda R¹  Halami Pm¹  Sachindra Nm²  Bhaskar N² 

[1] ,Central Food Technological Research Institute Department of Meat Fish and Poultry Technology ,India

关键词: Lactic acid bacteria;    fish waste;    E. faecalis NCIM 5367;    P. acidilactici NCIM 5368;    lipids;   

DOI  :  10.1590/S1517-83822011000400039

来源: SciELO

【 摘 要 】

Proteolytic and/or lipolytic lactic acid bacteria (LAB) were isolated from visceral wastes of different fresh water fishes. LAB count was found to be highest in case of visceral wastes of Mrigal (5.88 log cfu/g) and lowest in that of tilapia (4.22 log cfu/g). Morphological, biochemical and molecular characterization of the selected LAB isolates were carried out. Two isolates FJ1 (E. faecalis NCIM5367) and LP3 (P. acidilactici NCIM5368) showed both proteolytic and lipolytic properties. All the six native isolates selected for characterization showed antagonistic properties against several human pathogens. All the native isolates were sensitive to antibiotics cephalothin and clindamycin; and, resistant to cotrimoxazole and vancomycin. Considering individually, P. acidilactici FM37, P. acidilactici MW2 and E. faecalis FD3 were sensitive to erythromycin. The two strains FJ1 (E. faecalis NCIM 5367) and LP3 (P. acidilactici NCIM 5368) that had both proteolytic and lipolytic properties have the potential for application in fermentative recovery of lipids and proteins from fish processing wastes.

【 授权许可】

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