Triple inoculation with Bradyrhizobium, Glomus and Paenibacillus on cowpea (Vigna unguiculata [L.] walp.) development

Brazilian Journal of Microbiology

André Suêldo Tavares De Lima¹  Terezinha Ferreira Xavier¹  Cláudia Elizabete Pereira De Lima¹  José De Paula Oliveira¹  Adália Cavalcanti Do Espírito Santo Mergulhão¹  Márcia Do Vale Barreto Figueiredo¹ 

[1] ,Instituto Agronômico de PernambucoRecife PE ,Brasil

关键词: arbuscular mycorrhiza;    biological nitrogen fixation;    PGPB;    rhizobia;   

DOI  :  10.1590/S1517-83822011000300010

来源: SciELO

【 摘 要 】

The use of microorganisms to improve the availability of nutrients to plants is of great importance to agriculture. This study aimed to evaluate the effect of triple inoculation of cowpea with arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria (PGPB) and rhizobia to maximize biological nitrogen fixation (BNF) and promote plant growth. The experiment was conducted in a greenhouse using cowpea plants (Vigna unguiculata L. Walp cv. IPA 206). The treatments included inoculation with strains of Bradyrhizobium sp. (BR 3267 and EI - 6) individually and as a mixture, an absolute control (AC) and mineral nitrogen control (NC), all combined with the presence or absence of native AMF (Glomus etunicatum) and PGPB (Paenibacillus brasilensis - 24) in a 5x2x2 factorial design. All treatments were replicated three times. Contrasts were performed to study the treatment of variables. Inoculation with Bradyrhizobium sp. (BR 3267 and EI - 6) and G. etunicatum favored nitrogen acquisition and phosphorus availability for the cowpea plants. Inoculation with P. brasilensis - 24 increased colonization by Bradyrhizobium sp. and G. etunicatum and promoted cowpea growth, while the nitrogen from symbiosis was sufficient to supply the plants nutritional needs.

【 授权许可】

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