Some optimal culture conditions for production of cyclosporin a by Fusarium roseum

Brazilian Journal of Microbiology

Ahmed A. Ismaiel¹  El-sayed A. El-sayed¹  Asmaa A. Mahmoud¹ 

[1] ,Zagazig University Faculty of Science Department of BotanyZagazig,Egypt

关键词: Fusarium roseum;    Cyclosporin A;    Fermentation conditions;   

DOI  :  10.1590/S1517-83822010000400033

来源: SciELO

【 摘 要 】

A suitable chemically defined culture medium was selected and some optimal conditions for the production of the highly immunosuppressive compound, cyclosporin A (Cyc A) are reported. Medium of the following composition was favorable for the production of Cyc A by Fusarium roseum: glucose, 30; NaNO3, 2; KH2PO4, 1; MgSO4.7H2O, 0.5 and KCL, 0.5 (g/l). Maximum productivity of Cyc A was achieved at pH 6.0 when 50 ml of the fermentation medium/250 ml flask, inoculated with five fungal agar discs (6 mm, diameter) of 7-days old F. roseum culture after incubation at 30 ºC at 120 rpm for 7 days.

【 授权许可】

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