Bacterial community analysis of the water surface layer from a rice-planted and an unplanted flooded field

Brazilian Journal of Microbiology

Ana Fernández Scavino¹  Javier Menes¹  Lucía Ferrando¹  Silvana Tarlera¹ 

[1] ,Universidad de la República Facultad de Química Facultad de Ciencias Montevideo,Uruguay

关键词: floodwater;    rice;    microbial community;    T-RFLP;   

DOI  :  10.1590/S1517-83822010000200023

来源: SciELO

【 摘 要 】

The bacterial communities in floodwater, from a rice-planted and an unplanted field were characterized at the beginning (flooding stage) and at the end (harvest stage) of the rice cropping cycle. Most probable number estimations and plate counts of aerobic and anaerobic heterotrophic bacteria and of several metabolic bacterial groups (methanogens, sulfate-reducers, anaerobic sulfur and nonsulfur phototrophs, denitrifiers and ammonifiers) were similar in rice and unplanted floodwater at both sampling times. The analysis of denitrifiers and methanogens by fluorescent in situ hybridization revealed a shift in the phylogenetic affiliation only of the former group in the rice-planted floodwater. Terminal restriction fragment length polymorphism of 16S rRNA gene amplicons indicated that the bacterial communities of the rice-planted and unplanted soils were consistently diverse and strongly influenced by the season.

【 授权许可】

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