Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S rRNA and lipL32 gene sequencing

Brazilian Journal of Microbiology

Kumaresan Vedhagiri²  Kalimuthusamy Natarajaseenivasan²  Shanmugarajan G. Prabhakaran²  Joseph Selvin²  Ramasamy Narayanan²  Yogesh S. Shouche¹  Paluru Vijayachari¹  Sivalingam Ratnam¹ 

[1] ,Bharathidasan University School of Life Sciences Department of MicrobiologyTamilnadu,India

关键词: Leptospirosis;    Leptospira borgpetersenii;    lipL32;    16S rRNA;   

DOI  :  10.1590/S1517-83822010000100022

来源: SciELO

【 摘 要 】

The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out species segregation. Seroprevalence was about 51.4 %, and the predominant serovars were Autumnalis, Javanica, Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and sequence based molecular phylogeny indicated these two isolates were Leptospira borgpetersenii.

【 授权许可】

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