Detection of enterotoxin genes of Staphylococcus sp isolated from nasal cavities and hands of food handlers

Brazilian Journal of Microbiology

V.l.m. Rall²  J.m. Sforcin²  V.c.m. Augustini²  M.t. Watanabe²  A. Fernandes Jr.²  R. Rall¹  M.g. Silva¹  J.p Araújo Jr.² 

[1] ,Universidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e ImunologiaBotucatu SP ,Brasil

关键词: Staphylococcal enterotoxins;    coagulase-negative staphylococci;    S. aureus;    food handler;   

DOI  :  10.1590/S1517-83822010000100011

来源: SciELO

【 摘 要 】

Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.

【 授权许可】

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