Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or hematopoietic stem cell transplantation patients

Brazilian Journal of Microbiology

Débora De Campos Dieamant²  Sandra Helena Alves Bonon²  Liliane Cury Prates¹  Vera Maria Santoro Belangelo¹  Erika R. Pontes¹  Sandra Cecília Botelho Costa² 

[1],Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica MédicaCampinas SP ,Brasil

关键词: cytomegalovirus;    pediatric transplantation;    gB genotypes;    PCR;    antigenemia;   

DOI  :  10.1590/S1517-83822010000100010

来源: SciELO

【 摘 要 】

A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone marrow transplantation were monitored. Twenty out of thirty-three (60.6%) patients demonstrated active HCMV infection. gB1 and gB2 genotypes were more frequent in this population. In this study, we observed that gB2 had correlation with reactivation of HCMV infection and that patients with mixture of genotypes did not show any symptoms of HCMV disease. Future studies has been made to confirm this.

【 授权许可】

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