Isolation and identification of Profenofos degrading bacteria

Brazilian Journal of Microbiology

Saadatullah Malghani¹  Nivedita Chatterjee¹  Hu Xue Yu¹  Zejiao Luo¹ 

[1] ,China University of Geosciences School of Environmental Studies Laboratory of Biogeology and Environmental Geology, Ministry of EducationHubei,China

关键词: Profenofos;    Organophosphate pesticide;    Gas Chromatography;    Bioremediation;   

DOI  :  10.1590/S1517-83822009000400021

来源: SciELO

【 摘 要 】

An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99% similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28º to 36 ºC. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g-1; profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90% within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.

【 授权许可】

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