Production of laccase by Pynoporus sanguineus using 2,5 - Xylidine and ethanol

Brazilian Journal of Microbiology

Viviane S. Valeriano¹  Anna Maria F. Silva¹  Mariângela F. Santiago¹  Maria T. F. Bara¹  Telma A. Garcia¹ 

[1] ,Universidade Federal de Goiás Faculdade de Farmácia Laboratório de EnzimologiaGoiânia GO ,Brasil

关键词: Laccase;    Pycnoporus sanguineus;    ethanol;    2;    5-xylidine;   

DOI  :  10.1590/S1517-83822009000400009

来源: SciELO

【 摘 要 】

Enzyme application in biotechnological and environmental processes has had increasing interest due to its efficiency, selectivity and mainly for being environmentally healthful, but these applications require a great volume of enzymes. In this work the effect of different concentrations of ethanol and 2,5 - xylidine on growth and production of laccase by Pycnoporus sanguineus was investigated. In a medium containing 200 mg.L-1 of 2,5 - xylidine or 50 g.L-1 of ethanol, the maximum activity of laccase was 2019 U.L-1 and 1035 U.L-1, respectively. No direct correlation between biomass and activity of laccase was observed for any of the inducers used during the tests. Ethanol concentrations, larger than or equal to 20 g.L-1, inhibited the radial growth of P. sanguineus. This study showed that ethanol, which has less toxicity and cost than the majority of the studied inducers, presents promising perspectives for laccase production by P. sanguineus.

【 授权许可】

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