Exo-polygalacturonase production by Bacillus subtilis CM5 in solid state fermentation using cassava bagasse

Brazilian Journal of Microbiology

Manas R. Swain¹  Shaktimay Kar¹  Ramesh C. Ray¹ 

[1] ,Central Tuber Crops Research InstituteBhubaneswar,India

关键词: Exo-polygalacturonase;    Bacillus subtilis CM5;    response surface methodology;    solid state fermentation;    cassava bagasse;    exo-poligalacturonase;    Bacillus subtilis CM5;    metodologia de superfície de resposta;    fermentação em estado sólido;    bagaço de mandioca;   

DOI  :  10.1590/S1517-83822009000300028

来源: SciELO

【 摘 要 】

The purpose of this investigation was to study the effect of Bacillus subtilis CM5 in solid state fermentation using cassava bagasse for production of Exo-polygalacturonase (exo-PG). Response surface methodology was used to evaluate the effect of four main variables, i.e. incubation period, initial medium pH, moisture holding capacity (MHC) and incubation temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected exo-PG production. The experimental results showed that the optimum incubation period, pH, MHC and temperature were 6 days, 7.0, 70% and 50ºC, respectively for optimum exo-PG production.

【 授权许可】

CC BY-NC   
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