Brazilian Journal of Microbiology | |
Exo-polygalacturonase production by Bacillus subtilis CM5 in solid state fermentation using cassava bagasse | |
Manas R. Swain1  Shaktimay Kar1  Ramesh C. Ray1  | |
[1] ,Central Tuber Crops Research InstituteBhubaneswar,India | |
关键词: Exo-polygalacturonase; Bacillus subtilis CM5; response surface methodology; solid state fermentation; cassava bagasse; exo-poligalacturonase; Bacillus subtilis CM5; metodologia de superfície de resposta; fermentação em estado sólido; bagaço de mandioca; | |
DOI : 10.1590/S1517-83822009000300028 | |
来源: SciELO | |
【 摘 要 】
The purpose of this investigation was to study the effect of Bacillus subtilis CM5 in solid state fermentation using cassava bagasse for production of Exo-polygalacturonase (exo-PG). Response surface methodology was used to evaluate the effect of four main variables, i.e. incubation period, initial medium pH, moisture holding capacity (MHC) and incubation temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected exo-PG production. The experimental results showed that the optimum incubation period, pH, MHC and temperature were 6 days, 7.0, 70% and 50ºC, respectively for optimum exo-PG production.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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