Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples

Brazilian Journal of Microbiology

Lester J. Pérez¹  Heidy Díaz De Arce¹ 

[1] ,Centro Nacional de Sanidad Agropecuaria Grupo de Virología San José de las Lajas La Habana ,Cuba

关键词: Aujeszky's disease;    pseudorabies infection;    PCR assay;    doença de Aujeszky;    pseudo-raiva;    ensaio de PCR;   

DOI  :  10.1590/S1517-83822009000300002

来源: SciELO

【 摘 要 】

Aujeszky's disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase chain reaction (PCR) assay are presented for the diagnosis of pseudorabies infection. This assay was based on the amplification of a highly conserved viral gD gene fragment. PCR products of the expected size were obtained from PRV strains. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The analytical sensitivity of the test was estimated to be 1.34 TCID50/50 uL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.

【 授权许可】

CC BY-NC   
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