Assessing the diversity of bacterial communities associated with plants

Brazilian Journal of Microbiology

Fernando Dini Andreote¹  João Lúcio Azevedo¹  Welington Luiz Araújo¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de GenéticaPiracicaba SP ,Brasil

关键词: Plant-bacteria interactions;    molecular techniques;    multivariate analysis;    endophytes;    rhizosphere;    interação planta-bactéria;    técnicas moleculares;    análise multivariada;    endofíticos;    rizosfera;   

DOI  :  10.1590/S1517-83822009000300001

来源: SciELO

【 摘 要 】

Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information about the development of traditional methods and a general overview about the new insights into bacterial communities associated with plants.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License