In vitro establishment and propagation of a Brazilian strain ofAnaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells

Brazilian Journal of Microbiology

Camila V. Bastos²  Lygia M. F. Passos¹  Maria Mercês C. Vasconcelos²  Múcio F. B. Ribeiro² 

[1] ,Universidade Federal de Minas Gerais Departamento de Parasitologia Belo Horizonte MG ,Brasil

关键词: IDE8 cells;    Anaplasma marginale;    in vitro culture;    IDE8 cells;    Anaplasma marginale;    cultivo in vitro;   

DOI  :  10.1590/S1517-83822009000200034

来源: SciELO

【 摘 要 】

A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines.

【 授权许可】

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