Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using immobilized cells

Brazilian Journal of Microbiology

Daniela C. Orsi¹  Haroldo Y. Kawaguti¹  Hélia H. Sato¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia de Alimentos Departamento de Ciência de AlimentosCampinas SP ,Brasil

关键词: cell immobilization;    glucosyltransferase;    Klebsiella sp;    palatinose;    response surface methodology;    imobilização celular;    glicosiltransferase;    Klebsiella sp;    palatinose;    metodologia de superfície de resposta;   

DOI  :  10.1590/S1517-83822009000100010

来源: SciELO

【 摘 要 】

The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%) were evaluated and the yield of palatinose was approximately 62.5%.

【 授权许可】

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