Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source

Brazilian Journal of Microbiology

Aurora Martínez-trujillo²  Juan S. Aranda¹  Carlos Gómez-sánchez¹  Blanca Trejo-aguilar¹  Guillermo Aguilar-osorio¹ 

[1] ,Technologic Institute for Higher Studies of Ecatepec Laboratory of Enzymatic Catalysis Ecatepec Estado de Mexico

关键词: Aspergillus flavipes;    pectinolytic enzymes;    constitutive enzymes;    inducible enzymes;    pectin;    Aspergillus flavipes;    enzimas pectinolíticas;    enzimas constitutivas;    enzimas indutíveis;    pectina;   

DOI  :  10.1590/S1517-83822009000100006

来源: SciELO

【 摘 要 】

Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase.

【 授权许可】

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