Identification of an antifungal metabolite produced by a potential biocontrol Actinomyces strain A01

Brazilian Journal of Microbiology

Cai Ge Lu²  Wei Cheng Liu¹  Ji Yan Qiu¹  Hui Min Wang²  Ting Liu¹  De Wen Liu¹ 

[1] ,China Agricultural University Department of Plant Pathology Beijing,China

关键词: Streptomyces lydicus;    natamycin;    fungalplant disease;    biological control;    Streptomyces lydicus;    natamicina;    doenças fúngicas em plantas;    controle biológico;   

DOI  :  10.1590/S1517-83822008000400020

来源: SciELO

【 摘 要 】

Actinomyces strain A01 was isolated from soil of a vegetable field in the suburb of Beijing, China. According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain A01 was identified as Streptomyces lydicus. In the antimicrobial spectrum test strain A01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as Fusarium oxysporum, Botrytis cinerea, Monilinia laxa, etc. However, no antibacterial activity was found. In pot experiments in greenhouse, the development of tomato gray mold was markedly suppressed by treatment with the fermentation broth of the strain A01, and the control efficacy was higher than those of Pyrimethanil and Polyoxin. A main antifungal compound (purity 99.503%) was obtained from the fermentation broth of strain A01 using column chromatography and HPLC. The chemical structural analysis with UV, IR, MS, and NMR confirmed that the compound produced by the strain A01 is natamycin, a polyene antibiotic produced by S. chattanovgensis, S. natalensis, and S. gilvosporeus, widely used as a natural biological preservative for food according to previous reports. The present study revealed a new producing strain of natamycin and its potential application as a biological control agent for fungal plant diseases.

【 授权许可】

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