In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil

Brazilian Journal of Microbiology

Daniel M. Aguiar²  Mitika K. Hagiwara¹  Marcelo B. Labruna¹ 

[1] ,, Universidade Federal de Mato Grosso Faculdade de Agronomia e Medicina Veterinária Departamento de Clínica Médica VeterináriaCuiabá MT ,Brasil

关键词: Ehrlichia canis;    Dog;    Molecular Characterization;    Ehrlichiosis;    Ehrlichia canis;    Cão;    Caracterização molecular;    Erliquiose;   

DOI  :  10.1590/S1517-83822008000300014

来源: SciELO

【 摘 要 】

An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.

【 授权许可】

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