Bacterial diversity characterization in petroleum samples from Brazilian reservoirs

Brazilian Journal of Microbiology

Valéria Maia De Oliveira²  Lara Durães Sette²  Karen Christina Marques Simioni²  Eugênio Vaz Dos Santos Neto¹ 

[1] ,Universidade Estadual de Campinas Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas Campinas SP ,Brasil

关键词: bacterial diversity;    community DNA;    cultivation-independent analysis;    petroleum;    16S rDNA;    Diversidade bacteriana;    comunidade de DNA;    análise independente de cultivo;    petróleo;    16S rDNA;   

DOI  :  10.1590/S1517-83822008000300007

来源: SciELO

【 摘 要 】

This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were also cloned and sequenced, aiming at the taxonomic affiliation of the community members. The fingerprints obtained allowed the direct comparison among the bacterial communities from oil samples presenting distinct degrees of biodegradation, as well as between the communities of formation water and oil sample from the non-biodegraded reservoir. Very similar DGGE band profiles were observed for all samples, and the diversity of the predominant bacterial phylotypes was shown to be low. Cloning and sequencing results revealed major differences between formation water and oil samples from the non-biodegraded reservoir. Bacillus sp. and Halanaerobium sp. were shown to be the predominant components of the bacterial community from the formation water sample, whereas the oil sample also included Alicyclobacillus acidoterrestris, Rhodococcus sp., Streptomyces sp. and Acidithiobacillus ferrooxidans. The PCR-DGGE technique, combined with cloning and sequencing of PCR products, revealed the presence of taxonomic groups not found previously in these samples when using cultivation-based methods and 16S rRNA gene library assembly, confirming the need of a polyphasic study in order to improve the knowledge of the extent of microbial diversity in such extreme environments.

【 授权许可】

CC BY-NC   
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