Comparison of three decontamination methods for Mycobacterium bovis isolation

Brazilian Journal of Microbiology

Simone Rodrigues Ambrosio²  Eugenia Márcia De Deus Oliveira¹  Cesar Alejandro Rosales Rodriguez²  José Soares Ferreira Neto²  Marcos Amaku² 

[1],Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva e Saúde AnimalSão Paulo SP ,Brasil

关键词: Mycobacterium bovis;    decontamination;    Petroff;    HPC;    Mycobacterium bovis;    descontaminação;    Petroff;    HPC;   

DOI  :  10.1590/S1517-83822008000200008

来源: SciELO

【 摘 要 】

Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.

【 授权许可】

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